Composite

Part:BBa_K3278004

Designed by: Ailin Zhou   Group: iGEM19_ShanghaiTech_China   (2019-10-13)


pdawn with J23100 promoter

Change one of the promoters of pDawn system lacIq to J23100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1802
    Illegal NheI site found at 1825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 178
    Illegal NgoMIV site found at 272
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 1059
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1167
    Illegal AgeI site found at 397
    Illegal AgeI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1620
    Illegal BsaI.rc site found at 508


[edit]
Categories
Parameters
None